1. 16S/18S/ITS Amplicon metagenomic sequencing

Microbial community mapping has long been limited by the experimental difficulties in traditional methodologies at cell levels, until sequencing technology comes onto the stage with growing throughput and shrinking cost. The subunit on 16S rRNA containing both highly conserved and hypervariable regions, firstly discovered in 1977 by Woese et al., is a perfect molecular fingerprint for prokaryotic organisms identification. Subsequently, similar structures on 18S rRNA were described and applied for eukaryotes identification.
Taking advantage of sequencing, these amplicons can be targeted based on the conserved parts and the hypervariable regions can be fully charaterised for microbial identification contributing to studies covering microbial diversity analysis, taxonomy, phylogeny, etc.

Sample requirement:

2. Shotgun metagenomic sequencing

Metagenome refers to a collection of total genetic material of a mixed community of organisms, such as environmental metagenome, human metagenome, etc. It contains genomes of both cultivatable and uncultivatable microorganisms. Metagenomics is a molecular tool used to analyse the mixed genomic materials extracted from environmental samples, which provides detailed information in species diversity and abundancy, population structure, phylogenetic relationship, functional genes and correlation network with environmental factors, etc.

Sample requirement: